Identifikasi Keragaman Genetik Gen Reseptor Hormon Pertumbuhan (GHR|Alu I) Pada Sapi Bali

Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effects on target tissues. The objective of the study was to estimate genetic diversity of the GHR|AluI gene in Bali, limousin, simmental and pesisir cattle. Genotyping was performed on 248 animals, including 162 Bali, 21 limousin, 17 simmental and 48 pesisir. Single nucleotide polymorphisms (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. Genotype frequencies of Bali cattle AA (0.988), GG (0.006) and AG (0.006) were evidenced for the GHR AluI monomorphism, but mostly different from limousin GG (0.667), AA (0.238) and AG (0.095), simmental AG (0.529), GG (0.471) and AA (0.000), pesisir AA (0.604), GG (0.375) and AG (0.021) were the evidence of polymorphism. Homozigosity (monomorphism) in Bali cattle might be affected by adaptability in extreme environmental conditions such as poor nutrition and improper management practices. It also could be affected by natural selection and phenotype plasticity phenomena

Green tea polyphenol-reduced graphene oxide: derivatisation, reduction efficiency, reduction mechanism and cytotoxicity

This paper reports on the derivatisation, reduction efficiency, reduction mechanism and cytotoxicity of green tea polyphenol-reduced graphene oxide (GTP-RGO). The reduction of graphene oxide (GO) at 90°C using a weight ratio (WR) of GTP/GO=1 resulted in the production of a stable GTP-RGO dispersion in aqueous media, as indicated by the results of ultravioletvisible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA) and the measurement of zeta potential and electrophoretic mobility. In addition, the results from UV-Vis spectroscopy and X-ray photoelectron spectroscopy (XPS) analysis indicated the comparable reduction ability of GTP relative to the standard reducing agent, hydrazine (N2H4). The removal mechanism of epoxy group from GO via reduction reaction with GTP was investigated by implementing hybrid functional method of Becke-3-parameters-Lee-Yang-Parr (B3LYP)using Gaussian 09 software. The energy and frequency calculations showed that the GO reduction using GTP was more spontaneous and relatively took place faster than the reduction using N2H4, as evidenced by higher entropy change (ΔS) (0.039 kcal/mol·K) and lower Gibbs free energy (ΔG) barrier (58.880 kcal/mol).The cytotoxicities of GO and GTP-RGO samples were evaluated against human colonic fibroblasts cells (CCD-18Co). The GO sample was determined to be toxic even at low concentration (6.25 μg/mL), while the GTP-RGO sample possesses notably low toxicity at the same concentration. The cell culture experiments revealed that the incorporation of GTP led to a decrease in the toxicity of GTP-RGO samples

Koordinasi antara Kepala Sekolah dan Pengawas dalam Pelaksanaan Supervisi Akademik

The purpose of this study was to discribe how the general objective of the research is to describe the coordination between principal and supervisor in the implementing of instructional supervision is in the Elementary Schools of Karang Tinggi Sub District, Central Bengkulu Regency. The result of the study The result of the research showed that the coordination between principal and supervisor in the implementation of instructional supervision. That can be seen from the absence of coordination between principal and supervisor in the arrangement of instructional supervision planning program, there is no coordination intensity between principal and supervisor in the implementation of academic supervision, the coordination of monitoring and evaluating between principal and supervisor has not run optimally, and there is no coordination of result follow-up between principal and supervisor in the implementation of academic supervision

Pengelolaan Pembelajaran Bahasa Inggris Bidang Keterampilan Menulis

The objective of this research is to describe the instructional management of English in writing skill at Junior High School Number 01 South Bengkulu. The method of this research was qualitative research. The data were collected by conducting personal interview, observations and documentation study. Then, data analysis used qualitative technique. The result of this study showed that (1) in planning of learning of English writing skill, it was done well by English teachers, (2) in teaching learning process (implementation), English teachers used various and interesting methods and media. (3) in evaluating and supervision the learning management, teacher had found appropriate method of measuring the students\u27 achievement, (4) in feedback of teaching learning process was done by doing remedial and enrichment

Hubungan antara Stres Kerja dan Motivasi Berprestasi dengan Kinerja Guru

The purpose of this research was to describe the correlation between work stress and achieved motivation with teacher\u27s perfomance at the junior high school teachers at Manna East of Bengkulu. The method of research was correlational. The data was collected by using questionnaire, the data analysis was correlation, linear regression, and double regression to examine the hypotesis. The results of this research were (1) There was significant relation between work stress and teachers perfomance; (2) There was significant relation between achieved motivation and teachers perfomance; (3) There was together relation between work stress and achieved motivation to the teachers perfomance. According to the first and the second hyphothesis, it was conversed to the third hyphotesis, it was estimated that work stress followed by achieved motivation will increase the teachers perfomance